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Image Search Results
Journal: Vaccines
Article Title: Immune Correlates of Disseminated BCG Infection in IL12RB1-Deficient Mice
doi: 10.3390/vaccines10071147
Figure Lengend Snippet: The evaluation of PPD-specific immune response. Peritoneal macrophages were added to ELISPOT plates to enhance antigen presentation. ( a , d ) Representative images of spots formed after being stimulated with APC only, APC+PPD, or PMA+ionomycin. Numbers of PPD-specific IFN-γ secreting cell were compared between Il12rb1 −/− and C57BL/6 mice after being inoculated with BCG, either subcutaneously ( b ) or intranasally ( c ). The comparisons of the average optical intensities of spots stimulated by PPD between Il12rb1 −/− and C57BL/6 mice after being inoculated with BCG, either subcutaneously ( e ) or intranasally ( f ). ( g , h ) showed the comparisons of IFN-γ secreting cell numbers and the average optical intensities of spots between Il12rb1 −/− and C57BL/6 mice after PMA+ionomycin stimulation. ( i ) The cell culture supernatant in the wells of ELISPOT plates was collected for CBA assay. Levels of TNF-a were compared between Il12rb1 −/− and C57BL/6 mice inoculated with BCG, either subcutaneously or intranasally. All the above data were shown as mean ± SD. Statistical analysis were performed by the method two-tailed t -test (*, p < 0.05, **, p < 0.01, ***, p < 0.001). PPD-specific binding antibodies were detected by ELISA. Optical density (OD) values were compared between Il12rb1 −/− and C57BL/6 mice ( j ). Data are shown as mean ± SD. Statistical analyses were performed by the two-way ANOVA method (*, p < 0.05, **, p < 0.01).
Article Snippet: An in-house ELISA protocol was developed using purified
Techniques: Enzyme-linked Immunospot, Immunopeptidomics, Cell Culture, Two Tailed Test, Binding Assay, Enzyme-linked Immunosorbent Assay